By B.G. Belenkii, L.Z. Vilenchik
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Extra resources for Chromatography Library. Modern Liquid Chromatography of Macromolecules
118) For packed columnsA = 1 + 2 and for open capillary columnsA = 0. It should be taken into account that the value o f N in eqn. 1 18 is determined by the aims of chromatographic separation on the basis of the required values of K R , $ or c2 / O estimated by eqns. 104 respectively. The first step in optimization is the choice of the required values made by using these equations. ) parameters of the process and at a given value of N. When optimization of efficiency N is carried out, it is desirable to choose the operating parameters in such a manner as to obtain the required high value o f N .
Graphic illustration of the parameters of linear calibration dependence. The cz/VR ratio in eqn. 104) It is clear that the higher the value of cl/cz, the higher should be the value o f N i n order to satisfy the chosen efficiency criterion. 105) can be used. 97. 106) Let us evaluate their values for two ranges of molecular weights M E [ lo2 ; lo'] and M E [lo2; lo6] differing in width, and assume for simplicity that Vo/Vp is unity. 12 shows that coefficient B in eqn. 105 is inversely proportional to the width of the range of log M.
6. 1. introduction The main practical purpose of chromatography is the separation (at some grade of purity) of a mixture of substances for analysis or preparative fractionation. In the case of 22 GENERAL THEORY OF CHROMATOGRAPHY analysis two problems should be solved: the identification of chromatographic peaks and their quantitative interpretation. The difficulty lies in the fact that the separation of components during chromatography is always accompanied by spreading. Hence, these conflicting factors (separation and spreading) should be taken into account in designing a Chromatographic system and choosing the conditions of operation.