Download Carbohydrate Metabolism - Part E by Willis A. Wood (Eds.) PDF

By Willis A. Wood (Eds.)

The seriously acclaimed laboratory regular, Methods in Enzymology, is likely one of the such a lot hugely revered courses within the box of biochemistry. when you consider that 1955, each one quantity has been eagerly awaited, often consulted, and praised through researchers and reviewers alike. The sequence includes a lot fabric nonetheless suitable at the present time - really an important ebook for researchers in all fields of lifestyles sciences

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Extra resources for Carbohydrate Metabolism - Part E

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Ui, Biochim. Biophys. Acta 124, 310 (1966). 4 W. W. Cleland, Biochim. Biophys. Acta 67, 104 (1963). 5 T. Sumi and M. Ui, Biochim. Biophys. Acta 276, 12 (1972). [8] PFK FROM OYSTER A D D U C T O R MUSCLE 39 [8] Phosphofructokinase from Oyster Adductor Muscle By KENNETH B. 1 ,~Oysters are excellent anaerobes, capable of surviving several weeks of anoxia with anaerobic glycogenolysis supplying the energy needs of the animal. In oyster adductor muscle, PFK is closely regulated by the adenylates, arginine phosphate, and pH and is a key site controlling carbon flux during the aerobic-anaerobic transition.

4 F. J. Ballard, Bh~chem. J. 98, 347 (1966). -G. Blume and E. Beutler, J. Biol. Chem. 246, 6507 (1971). 6 M. A. Schell and D. B. Wilson, J. Biol. Chem. 252, 1162 (1977). 7 M. R. Henrich, J. Biol. Chem. 239, 50 (1964). METHODS IN ENZYMOLOGY, VOL. 90 Copyright © 1982by AcademicPress. Inc. All rights of reproduction in any form reserved. 5) buffers with overlapping at the transition pH values. 5. Stability. When stored at - 2 0 ° in the presence of 1 mM DTT and 20-30% glycerol, glucokinase preparations are stable indefinitely.

The following h e x o s e s and pentoses were found to be inactive for Drosophila hexokinases: N - a c e t y l g l u c o s a m i n e , galactose, sorbitol, ribose, and arabinose. b The values in parentheses refer to Km values. e Concentration of the c o e n z y m e s was 5 m M . 0. After centrifugation to remove the denatured protein, the enzyme solution is subjected to preparative column isoelectric focusing. The column (Model 8100-2,440 ml, from LKB, Sweden) is routinely run for 48 hr at 1200 V in the presence of 2% ampholyte (pH 5 to 7).

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